| Milla Jovovich | ... Alice |
| Sienna Guillory | ... Jill Valentine |
| Oded Fehr | ... Carlos Olivera |
| Thomas Kretschmann | ... Maj. Cain |
| Sophie Vavasseur | ... Angie Ashford |
| Sandrine Holt | ... Terri Morales |
| Raz Adoti | ... Sgt. Peyton Wells |
|
| with |
| Jared Harris | ... Dr. Ashford |
| Mike Epps | ... L. J. |
|
| Matthew G. Taylor | ... Nemesis |
| Zack Ward | ... Nicholai Ginovaef |
| Iain Glen | ... Dr. Isaacs |
| Dave Nichols | ... Capt. Henderson |
| Stefan Hayes | ... Yuri Loginova |
| Geoffrey Pounsett | ... Mackenzie |
| Shaun Austin-Olsen | ... Priest |
| Geny Walters | ... Mrs. Priest |
| Megan Fahlenbock | ... Marla Mapes |
| Robert Morelli | ... Director |
| Aaron Abrams | ... Assistant |
| Chris Benson | ... Lance Halloran |
| Martin Martinuzzi | ... Overworked Detective |
| Brad Borbridge | ... Overworked Sergeant |
| Frank Chiesurin | ... Sniper |
| Ron Benjamin | ... Bum |
| Kevin Hare | ... Home Owner / Scientist |
| Heather Dick | ... Older Woman |
| Alex Courey | ... Suit #1 |
| John Detoro | ... Suit #2 |
| Billy Khoury | ... Suit #3 |
| Sava Drayton | ... Suit #4 |
| Nicole Ann St. Hill | ... Undead Gaudy Hooker |
| Ricardo Betancourt | ... Burly Cop #1 |
| Ted Ludzik | ... Burly Cop #2 |
| Michelle Latimer | ... Tech #1 |
| Catherine Burdon | ... Tech #2 |
| Rebecca Sims | ... Undead Little Girl |
| Christopher Weedon | ... Undead Little Boy #1 |
| Billy Parrott | ... Security Guard |
| Derek Asland | ... Featured Graveyard Undead #1 |
| Richard Harte | ... Featured Graveyard Undead #2 |
| Simon Girard | ... Chopper Pilot #1 |
| Dean Copkov | ... Chopper Pilot #2 |
| Jeff White | ... Chopper Pilot #3 |
| Tim Burd | ... Refugee #1 |
| Natalie Charles | ... Refugee #2 |
| Pierre Henry | ... Screaming Soldier |
| Jim Codrington | ... Lieutenant |
| Deborah Valente | ... Tech |
| Kelly Fiddick | ... Reflection Undead |
| Malcolm Xerxes | ... Featured City Hall Undead #1 |
| Michy Gustavia | ... Featured City Hall Undead #2 |
| Shane Mackinnon | ... Featured City Hall Undead #3 |
| Ed Fielding | ... Featured City Hall Undead #4 |
| Stephen Hart | ... Featured City Hall Undead #5 |
| Sandi Stahlbrand | ... Reporter #1 |
| Chris Marren | ... Guard |
| Tom Gerhardt | ... Zombie Dad |
| Amber Marshall | ... Daughter |
| Venice Grant | ... Undead Stripper #1 |
| Ana Danilina | ... Undead Stripper #2 |
| Ingrid Johansson | ... Featured IV Woman Undead |
| Jeffrey Weedon | ... Undead Little Boy #2 |
| Melanie Tonello | ... Featured Undead Kid #1 |
| Annabel Chu | ... Featured Undead Kid #2 |
| Joey Jefferson | ... Featured Undead Kid #3 |
| Heather Braaten | ... Featured Rooftop Undead #1 |
| Richard Harte | ... Featured Rooftop Undead #2 |
| Joey Beck | ... Featured Rooftop Undead #3 |
| Eric Morgan | ... Featured City Walker #1 |
| Gene Pyrz | ... Featured City Walker #2 |
| Torquil Colbo | ... Featured City Walker #3 |
| Joan Moore | ... Featured City Walker #4 |
| Peter C. Wylde | ... Featured City Walker #5 |
| Garry Geddes | ... Featured City Walker #6 |
| Dionne Renee | ... Featured City Walker #7 |
| Janessa Crimi | ... Featured Undead Kid #4 |
| Dan Ellery | ... Featured Surplus Undead #1 |
| Sharon Moore | ... Featured Surplus Undead #2 |
| Derek Aasland | ... Featured Surplus Undead #3 |
| Jo Chim | ... Network Anchor #1 |
| Bobby Prochaska | ... Network Anchor #2 |
| Jazz Mann | ... Reporter #2 |
| Larissa Gomes | ... Reporter #3 |
| Gregory White | ... Lab Technician |
| Roman Podhora | ... Leader Umbrella Guard |
| Brian King | ... Umbrella Gate Guard |
| Rob Hall | ... Featured School Undead |
|
| Flashbacks from "Resident Evil" |
| Eric Mabius | ... Matt Addison |
